Pharmaseed team has a proven expertise in conducting stroke preclinical studies. Our comprehensive program includes a focus on neuroprotection, angiogenesis, pre-conditioning and rehabilitation.
We have a wide variety of stroke capabilities that enable the evaluation of primary and secondary outcomes through a number of induction options.
We provide guidance regarding which models (species, transient or permanent ischemic stroke models) would be the most appropriate for your technology.
Here are a few examples of our capabilities. If the model you need is not presented here, do not hesitate to contact us to learn how we can tailored our program to your specific R&D needs.
Our ischemic stroke models include well-established models such as focal cerebral ischemia, a widely used stroke injury model to mimic the pathophysiology of human stroke. Focal cerebral ischemia is induced via middle cerebral artery occlusions. These occlusions can be transient (reperfusion of the ischemic tissue) or permanent (no reperfusion).
We provide stroke models for the following species
The evaluation of stroke-induced injuries includes:
Our capabilities allow us to focus on neurodegeneration/neuroprotection, angiogenesis, pre-conditioning and rehabilitation.
Trans-cranial laser Doppler – analysis of cortical blood flow
Cerebral Imaging and Histology
Available histology analyses:
- Neuroinflammation assesments (NeuN, Iba1, Mac2)
- Astrocytes distribution marker (GFAP)
- Post-stroke angiogenesis (CD34, in mouse)
- Neo-angiogenesis evaluation (SMA, in rat)
- Neurogenesis (BrdU)
- Synaptic plasticity ( synaptophysin)
- Trophic factors (BDNF)
Stroke Behavioral Tests:
- Adhesive removal test
- Staircase skilled reaching
- Stepping test
- Neurological score
- Forelimb placing test
- Cylinder test
- Body Swing test
- Beam walking test
- Rota-Rod test
- Water maze
- Radial maze
- Social Recognition test
- Object Recognition test
Neurological score assessment in Pharmaseed laboratory