Complementary Ischemic Models

Ex-Ovo Computerized HET-CAM Assay

The HET CAM model is an ex vivo model for angiogenesis and anti-angiogenesis evaluation within days.

On day 3 of development, a fertilized egg was carefully opened over a tissue culture Petri dish. On day 5 an anti-angiogenic agent was applied onto the CAM using O-ring. Scoring of angiogenesis was carried out on day 12.

Graphical representation demonstrates the rate of angiogenesis (increase of blood vessels formation in the treated region) relative to a control group.

 

 

Endothelial Tube Formation assay

 

HUVEC-C cells were incubated at 37C and tube formation was observed for a period of 20h. Medium with and without Endothelial Cell Growth Supplement (ECGS) was used as positive control or negative control respectively.

 

Modified corneal pocket  model

Measurement of angiogenesis utilizing the experimental corneal angiogenesis rabbit model.